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human collagen type iv solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human collagen type iv solution
    Human Collagen Type Iv Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+type+iv/Collagen/pm41949496-380-8-17
    Average 99 stars, based on 1 article reviews
    human collagen type iv solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Cerebral Proteomic Changes in the rTg-D Rat Model of Cerebral Amyloid Angiopathy Type-2 With Cortical Microhemorrhages and Cognitive Impairments.
    Article Snippet: .. Staining for fibrillar amyloid was performed either using Amylo-Glo as described by the manufacturer (Biosensis Inc., Thebarton, South Australia) or staining with thioflavin S. The following antibodies were used for immunohistochemical analysis: rabbit polyclonal antibody to collagen type IV to visualize cerebral vessels (1:100; ThermoFisher, Rockford, IL); mouse monoclonal antibody to smooth muscle cell α actin (1:200, NBP 2-33006, Novus Bio); and antibody to HTRA1 (1:200, MAB2916, R&D Systems). ..

    Article Title: Cerebral Proteomic Changes in the rTg-D Rat Model of Cerebral Amyloid Angiopathy Type-2 With Cortical Microhemorrhages and Cognitive Impairments
    Article Snippet: .. Staining for fibrillar amyloid was performed either using Amylo-Glo as described by the manufacturer (Biosensis Inc., Thebarton, South Australia) or staining with thioflavin S. The following antibodies were used for immunohistochemical analysis: rabbit polyclonal antibody to collagen type IV to visualize cerebral vessels (1:100; ThermoFisher, Rockford, IL); mouse monoclonal antibody to smooth muscle cell α actin (1:200, NBP 2-33006, Novus Bio); and antibody to HTRA1 (1:200, MAB2916, R&D Systems). ..

    Immunohistochemical staining:

    Article Title: Cerebral Proteomic Changes in the rTg-D Rat Model of Cerebral Amyloid Angiopathy Type-2 With Cortical Microhemorrhages and Cognitive Impairments.
    Article Snippet: .. Staining for fibrillar amyloid was performed either using Amylo-Glo as described by the manufacturer (Biosensis Inc., Thebarton, South Australia) or staining with thioflavin S. The following antibodies were used for immunohistochemical analysis: rabbit polyclonal antibody to collagen type IV to visualize cerebral vessels (1:100; ThermoFisher, Rockford, IL); mouse monoclonal antibody to smooth muscle cell α actin (1:200, NBP 2-33006, Novus Bio); and antibody to HTRA1 (1:200, MAB2916, R&D Systems). ..

    Article Title: Cerebral Proteomic Changes in the rTg-D Rat Model of Cerebral Amyloid Angiopathy Type-2 With Cortical Microhemorrhages and Cognitive Impairments
    Article Snippet: .. Staining for fibrillar amyloid was performed either using Amylo-Glo as described by the manufacturer (Biosensis Inc., Thebarton, South Australia) or staining with thioflavin S. The following antibodies were used for immunohistochemical analysis: rabbit polyclonal antibody to collagen type IV to visualize cerebral vessels (1:100; ThermoFisher, Rockford, IL); mouse monoclonal antibody to smooth muscle cell α actin (1:200, NBP 2-33006, Novus Bio); and antibody to HTRA1 (1:200, MAB2916, R&D Systems). ..

    Derivative Assay:

    Article Title: Ultrasonically targeted destruction of SDF-1 nanobubbles carrying mesenchymal stem cells improves renal tubulointerstitial fibrosis.
    Article Snippet: Background and purpose: Renal tubulointerstitial fibrosis (RTF) is an irreversible pathological change that occurs during the end-stage of chronic kidney disease.. As a promising new approach, stem cell therapy offers the potential to reverse or ameliorate RTF by promoting cell proliferation in target tissues or through the secretion of relevant substances.. However, mesenchymal stem cells (MSCs) that home to renal tissues have reduced proliferation and vitality and a short retention time in the kidneys, which are key factors affecting therapeutic outcomes.

    Reverse Transcription:

    Article Title: Corneal endothelium ECM therapeutic medicaments
    Article Snippet: RNEasy (QIAGEN, Catalog number: 74106) was used in extraction of the total RNA from cells. .. The extracted RNA was subjected to a reverse transcription reaction (42° C., 60 minutes) with ReverTra Ace (TOYOBO Co., Ltd., Catalog number: TRT-101), and collagen type I, collagen type IV, and Fibronectin were amplified with a reaction reagent TaqMan Fast Advanced mastermix (Applied Biosystems) using GAPDH as an internal standard. .. Probes (a labeled primer set available from Applied Biosystems) described below was used in the PCR reaction, which was performed in the StepOneTM (Applied Biosystems) real-time PCR system.

    Amplification:

    Article Title: Corneal endothelium ECM therapeutic medicaments
    Article Snippet: RNEasy (QIAGEN, Catalog number: 74106) was used in extraction of the total RNA from cells. .. The extracted RNA was subjected to a reverse transcription reaction (42° C., 60 minutes) with ReverTra Ace (TOYOBO Co., Ltd., Catalog number: TRT-101), and collagen type I, collagen type IV, and Fibronectin were amplified with a reaction reagent TaqMan Fast Advanced mastermix (Applied Biosystems) using GAPDH as an internal standard. .. Probes (a labeled primer set available from Applied Biosystems) described below was used in the PCR reaction, which was performed in the StepOneTM (Applied Biosystems) real-time PCR system.

    Isolation:

    Article Title: Nicotinamide derivatives protect the blood-brain barrier against oxidative stress.
    Article Snippet: Nicotinamides play a crucial role in energy metabolism and maintenance of the redox homeostasis counteracting oxidative stress and elevated reactive oxidative species (ROS) in human cells.. The levels of nicotinamides decline with age and are associated with various pathologies, including ones linked with the blood-brain barrier disorder.. Therefore, the investigation of the bioactivity of synthetic nicotinamide derivates (NAs) and evaluation of their potential to protect the blood-brain barrier (BBB) from oxidative stress is emerging as an important new strategy.

    Cell Culture:

    Article Title: Nicotinamide derivatives protect the blood-brain barrier against oxidative stress.
    Article Snippet: Nicotinamides play a crucial role in energy metabolism and maintenance of the redox homeostasis counteracting oxidative stress and elevated reactive oxidative species (ROS) in human cells.. The levels of nicotinamides decline with age and are associated with various pathologies, including ones linked with the blood-brain barrier disorder.. Therefore, the investigation of the bioactivity of synthetic nicotinamide derivates (NAs) and evaluation of their potential to protect the blood-brain barrier (BBB) from oxidative stress is emerging as an important new strategy.

    Activation Assay:

    Article Title: Harmony in Healing: Investigating Platelet-Rich Plasma Activation during Acetylsalicylic Acid Treatment
    Article Snippet: .. The activation of PRP was performed by adding autologous thrombin and CaCl 2 (described above), collagen type I, or collagen type IV (final concentration 4 μg) (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Concentration Assay:

    Article Title: Harmony in Healing: Investigating Platelet-Rich Plasma Activation during Acetylsalicylic Acid Treatment
    Article Snippet: .. The activation of PRP was performed by adding autologous thrombin and CaCl 2 (described above), collagen type I, or collagen type IV (final concentration 4 μg) (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Binding Assay:

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis.
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 ◦C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 °C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.

    Blocking Assay:

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis.
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 ◦C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 °C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.

    Membrane:

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis.
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 ◦C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 °C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.

    Incubation:

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis.
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 ◦C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.

    Article Title: Balance between Pro- and Antifibrotic Proteins in Mesenchymal Stromal Cell Secretome Fractions Revealed by Proteome and Cell Subpopulation Analysis
    Article Snippet: .. Non-specific binding sites were blocked using 5% BSA in TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for 1 h. After blocking, the membrane was incubated with primary antibodies specific for ALIX (Abcam, Cambridge, UK; dilution 1/1000), beta-tubulin (Abcam, Cambridge, UK; dilution 1/1000), fibronectin (Abcam, Cambridge, UK; dilution 1/2000), HSP70 (Biocat, Heidelberg, Germany; dilution 1/1000), CD63 (Merck Millipore, Burlington, MA, USA; dilution 1/1000), CD81 (BioLegend, San-Diego, CA, USA; dilution 1/500), collagen type I (Abcam, Cambridge, UK; dilution 1/1000), collagen type IV (Thermo Fischer Scientific, Waltham, MA, USA; dilution 1/1000), or laminin (Abcam, Cambridge, UK; dilution 1/2000) in the blocking solution overnight at +4 °C. .. The membrane was then washed with TBST and incubated with secondary antibodies conjugated with horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) in the blocking solution.



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